1D and 2D NMRcharacterization of Sweitenia Mycrophylla gum

Abstract

Sweitenia mycrophyllagum (Mahoganygum) from Nigeria was characterized using standard procedures. Scanning electron microscopy (SEM), X-ray powdered diffractometry (XRDP), Fourier Transform Infrared (FTIR) and 1D and 2D Nuclear magnetic resonance (NMR) spectroscopy were used to characterized the gum from S.mycrophylla. SEM analysis suggested the gum has irregular particle size. The XRPD pattern of the gum showed no sharp peak which shows a completely armophous structure. FTIR spectrum showed band characteristics of O-H(3650-3500 cm-1), C-O-C (1632 cm-1), -COO (1429 cm-1) groups that are present in the structure of polysaccharide. NMR spectroscopy revealed that the gum is a galactomannan type polysaccharide which contains α-D galactopyranosyl unit linked to β-D-mannopyranosyl Unit at 0-6 with mannose/galactose ratio of 2.60 :1.20. Due to its mannose/galactose ratio, Sweitenia mycrophylla galactomannan could be a useful polysaccharide for food and pharmaceutical industry.