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|Title: ||Phenotypic and Genotypic Characterization of Isolates of Dermatophilus Congolensis from Cattle, Sheep and Goats in Jos, Nigeria|
|Authors: ||Shaibu, S.J|
polymerase chain reaction
multiple sequence analysis.
|Issue Date: ||2011|
|Publisher: ||African Journal of Microbiology Research|
|Series/Report no.: ||Vol. 5;No.5; Pp 467-474|
|Abstract: ||Dermatophilus congolensis is the causative agent of dermatophilosis an economically important
disease of livestock, and also an agent of zoonotic importance. The disease has been reported
worldwide, with a wide host range which includes domestic, wild and aquatic animals. This study was
therefore undertaken to characterize isolates of the organism from cattle, sheep and goats in Nigeria.
All the isolates, except two sheep isolates fermented glucose and sucrose. The whole cell protein
profiles of the isolates were similar at about 62 and 20 KDa, but different at other levels. The
polymerase chain reaction (PCR) of the partial 16SrRNA gene amplified all the isolates, but not other
organisms included. The multiple sequence alignment of the PCR amplicons sequences showed an
identity of between 98.5 and 100% across all the isolates. There was also a sequence similarity of
between 99.2 and 99.76% between the isolates and the partial sequence of the type strain of D.
congolensis DSM 44180T in the Genbank. Based on these techniques it may be concluded that all the
isolates are the same with minor differences which were not enough to speciate them.|
|Appears in Collections:||Veterinary Parasitology and Entomology|
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