Chemical and Mass Spectra Analysis of Fractions Obtained from Methanol Leaf Extract of Corymbia torelliana with Trypanosoma congolense Effect

Abstract

The use of drugs for the prevention and treatment of trypanosomiasis has been important for many decades, although trypanosomes have developed resistance to each drug introduced. This study is aimed at exploring Chromatography techniques to proffer solution for antitrypanosomal action from the leaves of Corymbia torelliana. The Chemical and Mass Spectra Analysis of compounds identified in the methanol leaves extract of Corymbia torelliana with Trypanosoma congolense effect was achieved using Column Chromatography, Thin Layer Chromatography and Gas Chromatography-Mass Spectral analysis. Column Chromatography was ran to obtain the separated fractions, which was pooled together for Thin Layer Chromatography. The GCMS analysis help to identify the compound by showing their molecular masses, fragmentation patterns, thermostatically molecular mass ions and the major ions of the fragments while the TLC was employed to locate the positions of various fractions as it untie the compounds identification in the system. These compounds are: 2(4H)- Benzofuranone, 5, 6, 7, 7atetrahydro- 4, 4,7a-trimethyl; 2-Naphthalene methanol, decahydro-alpha, alpha, 4a-trimethyl-8-methylene; Octanal, 2-(phenyl methylene); Hexadecanoic acid, methyl ester; Hexadecanoic acid; 1, 2, 3-Benzenetriol; 3, 5-Di-tertbutylphenol; Benzoic acid, 2-hydroxy-phenylmethyl ester; Hexadecanoic acid; Octadecanoic acid; 9,12,- Octadecadienoic acid methyl ester; 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl; Tetradecanoic acid, methyl ester; 11-Octadecenoic acid, methyl ester; 5-(p-tert-Butylphenoxymethyl)-3-(2-pyridyl)-2-oxazolidone and 1,2-Benzenedicarboxylic acid, dioctyl ester. The presence of these compounds suggest potentials of the leaves of Corymbia torelliana plant for possible uses as antitrypanosomal agent, anti-oxidant, anti-cancer, anti-tumour, antiinflammatory, insectifuge and remedy for mood and sleep disorder.