University of Jos Institutional Repository >
Veterinary Medicine >
Veterinary Public Health and Preventive Medicine >
Please use this identifier to cite or link to this item:
|Title: ||Detection of Inv a Virulence Gene by Polymerase Chain Reaction (PCR) in Salmonella Spp. Isolated from Captive Wildlife|
|Authors: ||Oladapo, Oludairo O.|
Kwaga, Jacob K.P.
Dzikwi, Asabe A.
|Issue Date: ||2013|
|Publisher: ||Bio-Genetic Journal|
|Series/Report no.: ||Vol. 1;No. 1; Pp 12-14|
|Abstract: ||Salmonella possesses genes that are responsible for virulence and establishment of infection in host cells. One of
such genes is inv A gene which is responsible for the invasion of the bacteria into host cells and is essential for
full virulence in Salmonella. The gene is specific for members of the genus Salmonella and have been said to
always be present in Salmonella spp. In this investigation, Salmonella spp. isolated from captive wildlife were
screened for inv A virulence gene. Eight isolates were tested by polymerase chain reaction (PCR) assay using
primers that amplify the 284 base pair fragment of the Salmonella inv A gene. Five out of the eight isolates were
positive for inv A gene. They were isolates from striped hyena, lion, spotted hyena, African hawk eagle and
peafowl while those from bateleur eagle, chimpanzee and another from African hawk eagle were negative.
Salmonella isolates carrying the inv A virulence genetic sequence are able to invade host cells and cause infection
while those without it may be unable to do that. The finding of this study also suggests that inv A gene may not
always be present in all Salmonella spp.|
|Appears in Collections:||Veterinary Public Health and Preventive Medicine|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.