University of Jos Institutional Repository >
Veterinary Medicine >
Veterinary Microbiology and Pathology >

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/2727

Title: Enterovirus-A76 of South-East Asian Ancestry in a Captive Chimpanzee (Pan troglodytes) in Jos, Nigeria
Authors: Oragwa, A. O.
George, U. E.
Faleye, T. O. C.
Adewumi, M. O.
Adeniji, J. A.
Keywords: Nonhuman primates
Issue Date: 2018
Publisher: Journal of Advances in Microbiology
Series/Report no.: Vol. 9;No. 3; Pp 1-8
Abstract: Background: Contact points between humans and nonhuman primates (NHPs) during the past decades have increased significantly in Africa. This provides opportunities for largescale emergence of novel virus types and species with unpredictable pathogenicity and clinical impacts. This study was designed to investigate and catalogue Enteroviruses (EVs) present in captive NHPs in Nigeria. Methods: Twenty-seven (27) fecal samples collected from captive NHPs in a Wild Life Park and Zoological garden at Jos, Nigeria in April 2016 were analyzed in this study. Samples were resuspended in a phosphate buffered saline (PBS)/chloroform mixture, and the clarified supernatant was subjected to RNA extraction, cDNA synthesis, a Panenterovirus 5I-UTR assay and three different enterovirus VP1 snPCR assays. All amplicons from the snPCR assays were sequenced, and enteroviruses identified using the enterovirus genotyping tool and phylogenetic analysis. Results: Eight (29.63%) (two each from Chimpanzees, Patas Monkey, Mona Monkey and Baboon) of the 27 samples were positive for the 5I-UTR assay. One (3.70%) of the 27 samples was positive for the enterovirus VP1 snPCR assays in addition to its positivity by 5ⁱ-UTR assay. The same sample happens to be one of the two samples from Chimpanzees that tested positive for the 5I-UTR assay, and it was subsequently identified as EV-A76 of South-East Asia ancestry. Conclusions: This study documents the first recorded attempt to detect and identify enteroviruses in NHPs in Nigeria. It also reports the first detection and identification of EV-A76 in Nigeria and particularly in a NHP. It is of utmost importance that the enterovirus VP1 assays be improved to enable detection of EVs that have been detected in NHPs but yet to be described in humans.
URI: http://hdl.handle.net/123456789/2727
ISSN: 2456-7116
Appears in Collections:Veterinary Microbiology and Pathology

Files in This Item:

File Description SizeFormat
Adewumi932018JAMB40008.pdf237.19 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback