Determination of the Biopesticidal and Antimicrobial Activity of Khaya Senegalensis Seed Oil Grown In Jos North Local Government Area of Plateau State, Nigeria

Abstract

Khaya senegalensis seed oil was evaluated for its effectiveness to control Callosobruchus maculatus on stored cowpea. The seed oil was extracted using steam distillation method of extraction. There was almost complete adult mortality of C. maculatus within 24 hrs after treatment with the seed oil at 1, 2 and 3ml/100g of cowpea, lower concentrations 0.2ml, 0.5ml, 0.8ml, required longer time for a notable effect on C. maculatus for all parameters examined, K. senegalensis seed oil has shown to have control over C. maculatus, suggesting that K. senegalensis seed oil has high potential for use as a botanical resource for control of C. maculatus. The antimicrobial activity was determined using Agar diffusion method with Gentamicin 10mg/ml serving as standard. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined using Tween 80 as diluents. The extract does not show appreciable antioxidant properties and the antibacterial analysis shows that the activity of the oil on organism isolate increase with increasing concentraton the oil inhibited Staphylococcus aureus, Salmonella typhi and E.coli. However the oil displayed bactericidal activities on organism isolate throughout the concentration. The effectiveness of the oil displayed bactericidal activities on organism isolate throughout the concentration. The effectiveness of the oil on organism isolate justified the claim among the traditional communities for treatment of ailment such as typhoid, skin rashes, boils and abscesses as plausible.