Characterization and Pharmaceutical Potentials of KhayaSenegalensis Seed Oil from Pankshin Plateau State

Abstract

The fruit of Khayasenegalensis plant, family Meliacease, were collected from Pankshin in the month of November and appropriately identified. The seed oil was extracted using steam distillation method, gas chromatography and mass spectroscopy (GC-MS) was performed, antioxidant analysis was done using DPPH according to Brand-William, Gallic and ascorbic acids served as standard. Anti-microbial activities was determined using Agar diffusion method with Gentamicin 10mg/ml serving as standard. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined with tween 80 as diluent. The GC-MS indicated the presence of urs-12-ene (C₃₀) and dodecanoic acid (C₁₂) etc. the extract does not show appreciable antioxidant properties and the antibacterial analysis shows that the activity of the oil on organism isolate increase with increasing concentration, the oil inhibited Staphylococcus aureus, Salmonella typhi and E.coli . The oil inhibited all the organism isolate at the minimum concentration of 31.25mg/ml. However the oil displayed bactericidal activities on organism isolate throughout the concentration. The effectiveness of the oil on organism isolate justified the claim among the traditional communities for treatment of ailment such as typhoid, skin rashes, boils and abscesses as plausible.