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http://hdl.handle.net/123456789/327
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Title: | Gene Expression of Aurora Kinases in Prostate Cancer and Nodular Hyperplasia Tissues |
Authors: | Nna, Emmanuel Madukwe, Jonathan Egbujo, Ejike Obiorah, Chris Okolie, Charles Echejoh, Godwin Yahaya, Amina Adisa, James Uzoma, Ijeoma |
Keywords: | Aurora kinase A Aurora kinase B Aurora kinase C |
Issue Date: | 13-Oct-2012 |
Publisher: | Medical Principle and Practice |
Abstract: | Objective: To measure the transcript levels of Aurora kinases
and compare them to their immunoreactivity patterns in
prostate tumors. Materials and Methods: A total of 26 cases
of prostate cancer (PCa) and 38 cases of benign nodular hyperplasia
(BPH) were sampled from archived formalin-fixed
paraffin-embedded tissues. Tissue sections were lysed, total
RNA extracted and cDNA made by random hexamer priming
while slide sections were immunostained for the kinases.
Normalized relative quantitation was performed for all the
kinases using real-time PCR on TaqMan chemistry. Results:
The immunoreactivity profile showed 15.4, 53.8 and 30.7%
positivity for Aurora kinases A, B and C in PCa cases, respectively,
while the positivity was 76.3, 73.7 and 84.2% for the
same kinases in BPH cases. The immunoreactivity was preponderant
on epithelial tissue compared to stromal component.
Conclusion: Aurora kinases were significantly overex- |
URI: | http://hdl.handle.net/123456789/327 |
Appears in Collections: | Histopathology
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