University of Jos Institutional Repository >
Veterinary Medicine >
Veterinary Physiology, Biochemistry and Pharmacology >

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/685

Title: PCR Detection and Identification of Avian Pasteurella Multocida in Clinical Samples Based on the KMT, Sequence
Authors: Yakubu, B
Haruna, E.S
Owolodun, O
Antiabong, J.F
Shaibu, S.J
Suleiman, A.B
Odugbo, M.O
Keywords: Southern hybridization,
Fowl cholera.
Issue Date: 2005
Publisher: Nigerian Veterinary Journal
Series/Report no.: Vol.27;No.1;Pp 39-47
Abstract: Polymerase Chain Reaction (PCR) was used for the detection and identification of Pasteurella multocida (PM)in poultry clinical samples with the KMT,SP/KMT,T, primer pair. A total of 54 clinical samples (liver, spleen, cloacae swabs)were assayed and finger prints of 14 samples corresponded with the 460bp expected band size. Results were unambiguous as finger prints were single and distinct. The sensitivity test of the KMT,SP/ KMT,T, primer pair gave 10⁻⁵ dilution (0.03025µg) as the limit of detection using ethidium bromide staining of amplified DNA in agarose gel electrophoresis. The PM-PCR was specific and reproduceble. Southern hybridization results confirmed the PM-PCR results and have therefore been shown to be a sensitive and reproducible confirmatory assay. The KMT, sequence based primers (KMT,SP/KMT,T,) proved sensitive and reliable to be used for the detection and identification of PM in clinical samples.
URI: http://hdl.handle.net/123456789/685
Appears in Collections:Veterinary Physiology, Biochemistry and Pharmacology

Files in This Item:

File Description SizeFormat
3503-11966-1-PB.pdf777.01 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback