University of Jos Institutional Repository >
Natural Sciences >
Plant Science and Biotechnology >

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/847

Title: Keratinolytic Activity of Cladosporium and Trichoderma Species Isolated From Barbers’ Landfill
Authors: Nwadiaro, Patience
Ogbonna, Abigail
Wuyep, Ponchang
Adekojo, Deborah
Keywords: Fungi
Human hair
Skim milk
Chicken feather
Issue Date: 30-May-2015
Publisher: International Journal of Biosciences | IJB |
Series/Report no.: Vol. 6;No.10; Pp 104-115
Abstract: Studies were carried out on the ability of filamentous fungi to degrade keratin based substrates (human hair and chicken feather) using their keratinase. Soil samples were collected at random from barbers’ landfills in Jos city, Nigeria and were screened using the hair baiting technique. Proteolytic ability of the fungal isolates was carried out using skim milk agar. Fungal strains that showed the highest activity following the diameter zones of clearance were further tested for keratinase activity in submerged fermentation (SmF) using basal mineral medium supplemented with human hair and chicken feather as sole sources of carbon and nitrogen. A total of 17 fungal species belonging to 10 genera were isolated with Aspergillus species the most dominant. Cladosporium cladosporioides and Trichoderma viride had the highest potential to hydrolyze skim milk casein with the peak period of 72h (77.1U/ml and 50.1U/ml) for C. cladosporioides and T. viride, respectively, after which there was a decline in enzyme production. The keratinase activity of the two species on both human hair and chicken feather were highly variable. C. cladosporioides and T. viride had keratinase activity of 6.3 U/ml and 37.5 U/ml after 72h and 96h, respectively, on human hair while for chicken feather medium, C. cladosporioides had keratinase activity of 35.5 U/ml after 96h and T. viride 37.5 U/ml after 72h. The spent medium containing chicken feather showed higher specific activity for keratinase as compared to the spent human hair medium. It is deduced that these fungi may have an important role in the degradation of keratin containing wastes in a natural environment.
URI: http://hdl.handle.net/123456789/847
ISSN: 2222-5234
Appears in Collections:Plant Science and Biotechnology

Files in This Item:

File Description SizeFormat
IJB-V6No10-p104-115.pdf532.95 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback